| Solution Information | help | |
| Enzyme: | Cyclin-dependent kinase 2 | |
| inhibitor: | BDBM84555 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | 8.0 | |
| Temp. Prep.: | 22 | |
| Comments: | GSK3-Beta | |
| pH Buffer 1: | MgCl2 | |
| Concentration: | 0.5 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 2: | Dithiothreitol | |
| Concentration: | 1.0 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 3: | ATP | |
| Concentration: | 0.5 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 4: | gamma-33 ATP | |
| Concentration: | 62.5 nCi per well | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 5: | Dimethyl sulfoxide | |
| Concentration: | 1.25% v/v | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 6: | Nonidet P40 | |
| Concentration: | 0.002% | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 7: | MnCl2 | |
| Concentration: | 0.5 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 8: | Mg acetate | |
| Concentration: | 2.5 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 9: | Biotinylated gsk3-beta substrate peptide | |
| Concentration: | 4 uM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
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